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KMID : 0613320050110010046
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Journal of the Korean Bone and Joint Tumor Soceity
2005 Volume.11 No. 1 p.46 ~ p.53
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Tumor Suppressive Effect of Zoledronic Acid on Human Osteosarcoma Cells in Vivo
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Kim Jae-Do
Jang Jae-Ho
Lee Young-Gu
Lee Dong-Won
Kwon Young-Ho
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Abstract
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Purpose: Bisphosphonates (BPs) are the analogues of endogenous pyrophosphates: they have been used in the treatment of skeletal diseases such as Paget’s disease, osteoporosis, and tumorinducing ostelysis, and are used in treatment of osteolytic metastasis of breast cancer recently. They are also used as one of the therapeutic agents for metastasis of prostatic cancer of which metastasis makes the mixed nature of osteolysis and ostegenesis. Although the action mechanism of BPs are well known for diseases with excessive osteoclastic bone resorption, the direct
effect of BPs has not been known yet. This study was intended to see the tumor suppression capability of Zoledronic acid(ZOL) using nude mouse with osteosarcoma.
Materials and Methods: MG-63 and HOS osteosarcoma cell lines were used and the transforemed MG-63-GFP and HOS-GFP cells, which were made for detection under fluorescent light, were subcutaneously injected to make osteosarcoma. The five 6-week male mice were used for the experiment at each group. After the injection, mice were cultivated until tumor pieces grow up to 3×3×3 mm3 and ZOL of 120 ug/kg was subcutaneously injected twice a week. Sizes of tumor were measured twice a week and photographed under fluorescent light.
Result: In in vivo test with HOS osteosarcoma cell lines, mean size of tumors was 2,520 mm3 in control group and was 131 mm3 in ZOL group ,which showed 94% of reduction comparing with the control ; with MG-63 osteosarcoma cell lines, mean size of tumors was 2,866 mm3 in control group and was 209 mm3 in test group with 72% of reduction (p<0.05).
Conclusion: In in vivo tests with nude mice, we suggest that ZOL has direct effect on osteosarcoma cells and it would be used as one of the therapeutic agents for osteosarcoma, especially to ZOL-sensitive osteosarcoma cells.
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KEYWORD
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Osteosarcoma, Nude mouse
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